15–16 Sept 2011
Paul Scherrer Institut, Villigen, Switzerland
Europe/Zurich timezone

Silver nanoparticles and synchrotron light-based nanotoxicology: new imaging modalities

15 Sept 2011, 13:17
2m
WSLA - Foyer (Paul Scherrer Institut, Villigen, Switzerland)

WSLA - Foyer

Paul Scherrer Institut, Villigen, Switzerland

Board: 14
Poster Poster Session I (Thursday) Poster session I and lunch

Speaker

Dr Alexandre Mantion (BAM Federal Institute for Materials Research and Testing)

Description

Silver nanoparticles belong to the most studied, produced and broadly distributed nanoparticles. Despite this, a critical investigation of their potential adverse effects on model living and ecological systems is still required. Along well-established biochemical tests, a reproducible and simple methodology to image their precise distribution in cells is missing. Their imaging in cells requires usually complex preparation like embedment/sectioning for electron microscopy or may have only a limited spatial resolution like in confocal Raman Microscopy. Often, studying the chemical composition and oxidation state of elements within a cell is a daring task. Our aim is thus to further exploit synchrotron radiation for nanotoxicology and contribute to develop complementary protocols for biological object imaging. To do this, we incubate a model macrophage cell line with our in house developed silver nanoparticles system and study the internal distribution of particles using ptychography, Scanning X Ray Transmission Microscopy (STXM), tomography and X ray absorption spectroscopy at the cSAXS and microXAS beamlines at SLS. We will present our first results in this poster.

Please specify the session

Imaging / Advancing Quantitative Chemical Imaging

Please specify poster or talk

poster

Primary author

Dr Alexandre Mantion (BAM Federal Institute for Materials Research and Testing)

Co-authors

Dr Ana Diaz (Paul Scherrer Institute) Dr Andrea Haase (BfR - Federal Institute for Risk Assessment) Dr Andreas Luch (BfR - Federal Institute for Risk Assessment) Andreas Taubert (Universität Potsdam and MPI Colloids and Interfaces) Dr Andreas Thünemann (BAM Federal Institute for Materials Research and Testing) Dr Daniel Grolimund (Paul Scherrer Institute) Dr Joan Vila (Advanced Photon Source, Argonne) Johanna Plendl (BfR - Federal Institute for Risk Assessment) Mrs Monika Sachtleben (Free University of Berlin, Department of Veterinary Medicine, Institute of Veterinary Anatomy) Philipp Graf (Universität Basel) Wolfgang Meier (Universität Basel)

Presentation materials

There are no materials yet.