Practical training

Mesophase preparation, crystallization plate set up and evaluation
 
Serial diffraction data collection with a microfocused beam
 
Serial data processing, selection and analysis
 



References to papers

Participants are strongly encouraged to read the following bibliography:

Caffrey M, Cherezov V. (2009) Crystallizing membrane proteins using lipidic mesophases. Nat Protoc. 4(5):706-31. (PDF)

Huang C-Y., Olieric V., Ma P., Howe N., Vogeley L., Liu X., Warshamanage R., Weinert T., Panepucci E., Kobilka B., Diederichs K., Wang M., Caffrey M.  (2016) In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures. Acta Cryst. D72, 93-112 (PDF)
(Movie 1) (Movie 2)(Movie 3)(Movie 4)
 
Diederichs, K. (2015) Crystallographic data and model quality. In
Ennifer, E. (Ed.), Nucleic Acid Crystallography: Methods and Protocols,
Methods in Molecular Biology 1320. New York, NY: Springer, 147-173. (PDF)
 
Karplus, P.A., Diederichs, K. (2015) Assessing and maximizing data
quality in macromolecular crystallography.
Curr. Opin. Struct. Biol. 34, 60-68. (PDF)

Assmann, G., Brehm, W. and Diederichs, K. (2016) Identification of rogue datasets in serial crystallography. J. Appl. Cryst. 49, 1021-1028. (PDF)
 
Diederichs K. (2017) Dissecting random and systematic differences between noisy composite data sets. Acta Cryst D73:286-293. (PDF)