Amyloid proteins meet physics: from X-rays and neutrons over electrons to magnetism

Monday 5 Sept 2022, 08:30 → 21:30 Europe/Zurich

The education center, SZ-OSGA/EG06 (Paul Scherrer Institute)

Anne Bonnin (PSI - Paul Scherrer Institut), Jinghui Luo (PSI - Paul Scherrer Institut)

Coffee chat and introduction to the symposium

Coffee chat and introduction to the symposium from 08:30-09:00 (Jinghui Luo, Anne Bonnin)

Visualising amyloid deposits by X-rays and electrons

09:00-09:30 Henning Stahlberg (EPFL, Electron diffraction & imaging)
Coherent electron diffractive imaging in single particle cryo-EM
09:30-09:50 Ana Diaz (PSI, cSAXS beamline, X-ray ptychography imaging)
X-ray nanotomography of biological tissues by ptychographic tomography
09:50-10:10 Anne Bonnin (PSI, Tomcat beamline, X-ray phase contrast imaging)
X-ray phase contrast tomography at the TOMCAT beamline:  a focus on brain imaging

1 Coherent Electron Diffractive Imaging in single particle cryo-EM

Speaker: Henning Stahlberg (EPFL, Electron diffraction and imaging)

2 X-ray nanotomography of biological tissues by ptychographic tomography

Speaker: Ana Diaz (PSI, cSAXS beamline, X-ray ptychography imaging)

3 X-ray phase contrast tomography at the TOMCAT beamline: a focus on brain imaging

Speaker: Anne Bonnin (PSI, Tomcat beamline, X-ray phase contrast imaging)

10:10 Coffee Break

Visualising amyloid deposits by X-rays and electrons

10:30-11:00 Peter Faller (University of Strasbourg, EPR & X-ray),
Copper bound to amyloid-beta: from structure and reactivity insights to its targeting as therapeutic approach
11:00-11:20 Armin Kleibert (PSI, SIM beamline, photo-emission electron microscopy)
Soft X-ray spectromicroscopy for nanoscale chemical characterization at the SIM Beamline of the SLS
11:20-11:40 Rebecca Sternke-Hoffmann (PSI,BIO)
Alanine switches the protein crystal formation to the amyloid fibril formation

4 Copper bound to amyloid-beta: from structure and reactivity insights to its targeting as therapeutic approach

Speaker: Peter Faller (University of Strasbourg, EPR & X-ray)

5 Soft X-Ray Spectromicroscopy for Nanoscale Chemical Characterization at the SIM Beamline of the SLS

Speaker: Armin Kleibert (PSI, SIM beamline, photo-emission electron microscopy)

6 Alanine switches protein crystal formation to amyloid fibril formation

Speaker: Rebecca Sternke-Hoffmann (PSI, BIO Department)

11:40 Lunch break at OASE

Studying amyloid dynamics and metal ions by X-rays and magnetism

13:00-13:30 Roland Riek (ETH Zurich, NMR),
The structural and dynamics landscape of proteins using NMR
13:30-13:50 Maarten Nachtegaal (PSI, SuperXAS, X-ray spectroscopy)
X-ray absorption spectroscopy provides insight in the oxidation state and local structure of metal sites
13:50-14:10 Jan Dreiser (PSI, Xtreme beamline, X-ray magnetic circular dichroism)
Insights into amyloid-copper interaction from soft X-ray absorption spectroscopy

7 The structural and dynamics landscape of proteins using NMR

Speaker: Roland Riek (ETH, NMR)

8 X-ray absorption spectroscopy provides insight in the oxidation state and local structure of metal sites

Speaker: Maarten Nachtegaal (PSI, SuperXAS, X-ray spectroscopy)

9 Insights Into Amyloid-Copper Interaction From Soft X-Ray Absorption Spectroscopy

Speaker: Jan Dreiser (PSI, Xtreme beamline for XMCD, X-ray magnetic circular dichroism)

14:10 Coffee Break

Studying amyloid dynamics and metal ions by neutrons and magnetism

14:30-15:00 Ayyalusamy Ramamoorthy (The University of Michigan, Ann Arbor, NMR),
Probing the effects of lipids and lipid membrane on amyloid aggregation
15:00-15:20 Jinming Wu (PSI, BIO)
Identifying the role of co-aggregation of Alzheimer’s Amyloid-β with amorphous protein aggregates of non-amyloid proteins 
15:20-15:40 Fanni Juranyi (PSI, FOCUS beamline, neutron spectroscopy)
Protein dynamics: what can we learn from neutron spectroscopy
15:40-16:00 Viviane Lütz Bueno (PSI, SANS beamline, neutron scattering)
Amyloid fibrils: sustainable building blocks for materials science

10 Probing the effects of lipids and lipid membrane on amyloid aggregation

Speaker: Ayyalusamy Ramamoorthy (The University of Michigan, Ann Arbor, NMR)

11 Identifying the role of co-aggregation of Alzheimer’s Amyloid-β with amorphous protein aggregates of non-amyloid proteins

Speaker: Jinming Wu (PSI, BIO Department)

12 Protein dynamics: what can we learn from neutron spectroscopy

Speaker: Fanni Juranyi (PSI, FOCUS, neutron spectroscopy)

13 Amyloid fibrils: sustainable building blocks for materials science

Speaker: Viviane Lütz Bueno (PSI, SANS, neutron scattering)

Ending discussions and Tour to SLS/SINQ

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