Speaker
Dr
Arwen Pearson
(Astbury Centre for Structural Molecular Biology, University of Leeds)
Description
The fundamental aim of structural biology is to understand the molecular basis of life. To achieve this, a high resolution description of how the system changes with time is required. Single time-averaged structures, for example those of cryotrapped intermediates, provide snap-shots of a reaction that yield considerable information. However, cryotrapping approaches are limited to the study of longer lived, metastable, states. To observe short-lived species a time-resolved approach is required.
We are developing a new approach to time-resolved crystallography that combines the new developments in microfocus beams and beamline automation with information from single crystal spectroscopy to study non-reversible enzyme reactions. Here we will present initial results using our test system, aspartate decarboxylase.
Primary author
Dr
Arwen Pearson
(Astbury Centre for Structural Molecular Biology, University of Leeds)
Co-authors
Ms
Briony Yorke
(University of Leeds)
Dr
Mike Webb
(University of Leeds)
Dr
Robin Owen
(Diamond Light Source)
