Speaker
Description
Life is thought to have appeared in the depth of the sea under high hydrostatic pressure (HHP). Indeed, it is known that the deep biosphere hosts a myriad of life forms thriving under high pressure conditions. However, the evolutionary mechanisms leading to their adaptation are still not known. Here, I will discuss the impact of HHP on various biological systems covering lipidic membranes, proteins and whole cells.
Neutron scattering is a well-suited tool to disentangle various structural and dynamical elements allowing biosystems to withstand such conditions. However, it requests also suitable high pressure equipment taking into account the particularities of these experiments [1-3]. Based on research over the last years, I will present new insights into high hydrostatic pressure adaptation [4-7].
1 J. Peters, M. Trapp, D. Hughes, S. Rowe, B. Demé, J.-L. Laborier, C. Payre, J.-P. Gonzales, S. Baudoin, N. Belkhier, E. Lelievre-Berna, High hydrostatic pressure equipment for neutron scattering studies of samples in solutions, High Pressure Research 32(1) (2011) 97–102.
[2] E. Lelièvre-Berna, B. Demé, J. Gonthier, J.P. Gonzales, J. Maurice, Y. Memphis, C. Payre, P. Oger, J. Peters, S. Vial, 700 MPa sample stick for studying liquid samples or solid-gas reactions down to 1.8 K and up to 550 K, Journal of Neutron Research 19 (2017) 77 – 84.
[3] J. Peters, M. Golub, B. Demé, J. Gonthier, C. Payre, J. Maurice, R. Sadykov, E. Lelièvre-Berna, New pressure cells for membrane layers and systems in solutions up to 100°C, J. Neutron Res. 20 (2018) 3 – 12.
[4] M. Salvador-Castell, M. Golub, N. Erwin, B. Demé, N.J. Brooks, R. Winter, J. Peters, P.M. Oger, Characterisation of a synthetic Archeal membrane reveals a possible new adaptation route to extreme conditions, Commun Biol 4(1) (2021) 653.
[5] L. Misuraca, B. Demé, P. Oger, J. Peters, Alkanes increase the stability of early life membrane models under extreme pressure and temperature conditions, Communications Chemistry 4 (2021) 24, 1 – 8.
[6] A. Caliò, C. Dubois, S. Fontanay, M.M. Koza, F. Hoh, C. Roumestand, P. Oger, J. Peters, Unravelling the Adaptation Mechanisms to High Pressure in Proteins, Int. J. Mol. Sci. 23 (2022) 8469, 1 – 17.
[7] N. Martinez, G. Michoud, A. Cario, J. Ollivier, B. Franzetti, M. Jebbar, P. Oger, J. Peters, High protein flexibility and reduced hydration water dynamics are key pressure adaptive strategies in prokaryotes, Sci Rep 6 (2016) 32816.
E-mail for corresponding author: jpeters@ill.fr