Speaker
Dr
valerie panneels
(Paul Scherrer Institute)
Description
Membrane proteins (MPs) represent key components of cell membranes, about one fourth of the human proteome and 60% of the drug targets. We are interested in learning about the different conformations a MP can adopt in response to interacting molecules. The best way to analyse this at the atomic level is to determine their 3D-structure by crystal X-ray diffraction. We aim at producing nanocrystals of MPs and of supramolecular complexes, especially those related to tubulin, for analysis with FELs. A range of techniques will have to be employed for nanocrystal detection. Nanocrystals may exhibit fewer lattice imperfections compared to larger crystals. The x-ray beam parameters needed to analyse nanocrystals by “diffract-and-destroy” serial crystallography will be discussed as well as the way to deliver the nanocrystals to the beam.
Primary author
Dr
valerie panneels
(Paul Scherrer Institute)
Co-authors
Dr
Ching-Ju Tsai
(Paul Scherrer Institute)
Prof.
Gebhard Schertler
(Paul Scherrer Institute)
Dr
Guido Capitani
(Paul Scherrer Institute)
Dr
Xiao-Dan Li
(Paul Scherrer Institute)
