Speaker
Description
Photosystem II (PSII) plays a critical role in water-splitting and oxygen evolution. X-ray crystallography has elucidated its atomic structure and structures of several intermediate states. However, these structures are in the crystalline state, and the structure of the final state remains unresolved. In this study, we analyzed the structure of PSII in solution at a resolution of 1.95 Å using single-particle cryo-electron microscopy (cryo-EM). The obtained structure is similar to the crystal structure, but regions susceptible to redox state changes exhibited electron beam damages at high doses. By reducing the number of movie frames of electron micrographs from 50 to 2 to lower the beam dose, the damages were minimized while the resolution was comparable (Fig. a). I will discuss the details of the electron beam-induced damages and their minimization in this presentation.
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References
[1] Kato K. et al. (2021) Commun Biol., 4, 382.
