Speaker
Description
Time-resolved pump-and-probe X-ray solution scattering experiments can probe
kinetics and dynamics of biological macromolecules in real time and under nearnative
conditions. A concern is radiation damage to the sample. One approach to
reduce radiation damage is to use mechanical devices to probe only with short X-ray
pulses. We used this approach to monitor intermediate states in calcium transport at
beamline ID09 at the ESRF 1,2. However, such experimental designs are delicate and
typically only available at dedicated time-resolved synchrotron beamlines, which
limits availability. An alternative approach is to use detector readout to obtain the
temporal resolution, which requires careful characterization of radiation damage. We
developed such an approach at the CoSAXS beamline at MAX IV Laboratory and
tracked kinetics and structural dynamics of the enzyme adenylate kinase 3.
References
[1] Ravishankar, H., Pedersen, M.N., Eklund, M., Sitsel, A., Li, C., Duelli, A., Levantino, M., Wulff, M.,
Barth, A., Olesen, C., Nissen, P., Andersson, M. (2020) Science Advances. 6(12), eaaz0981.
[2] Prabudiansyah, I., Orädd, F., Magkakis, K., Pounot, K., Levantino, M., Andersson, M. (2024)
Science Advances. 10(41), eadp2916.
[3] Magkakis, K., Orädd, F., Ahn, B., Da Silva, V., Appio, R., Plivelic, T., Andersson, M. (2024)
Structure. 32(9), P1519-1527.
