Conveners
Radiation Damage in Complementary Fields including Biological Imaging
- Daniele de Sanctis (ESRF)
High-resolution X-ray microscopy is used as a complementary approach to electron microscopy for non-destructive imaging of soft materials, including biological tissues, e.g. frozen hydrated [1] or heavy metal stained resin-embedded [2] brain tissues. For this purpose, photon energies above about 2 keV are used to penetrate tissues of about 100 micron thickness or more, for which samples...
Radiation damage remains a central challenge in structural biology, particularly when probing soft, disordered materials like hydrated proteins. In this presentation, I will discuss recent advances using X-ray Photon Correlation Spectroscopy (XPCS) to study radiation-driven dynamics in both hydrated protein powders [1] and dense protein solutions [2,3]. At cryogenic temperatures, we...
Time-resolved pump-and-probe X-ray solution scattering experiments can probe
kinetics and dynamics of biological macromolecules in real time and under nearnative
conditions. A concern is radiation damage to the sample. One approach to
reduce radiation damage is to use mechanical devices to probe only with short X-ray
pulses. We used this approach to monitor intermediate states in calcium...
