12th International Workshop on Radiation Damage to Biological Samples

Session

Radiation Damage in Temperature Controlled Crystallography

TEMP

5 Jun 2025, 11:00

Auditorium (Paul Scherrer Institut)

Conveners

Radiation Damage in Temperature Controlled Crystallography

• Vincent Olieric (PSI - Paul Scherrer Institut)

21. Mapping the Conformational Landscapes of Human Kinases with Multi-Temperature X-ray Crystallography

Michael THOMPSON (UC Merced)

05/06/2025, 11:00

Invited

Protein kinases are a large family of enzymes that regulate diverse cellular processes by transfering phosphate groups from ATP to their protein substrates. The enzymatic kinase domain (KD) represents a conserved fold, and KD phosphorylation, as well as interactions with cis-regulatory elements and other allosteric effectors, modulates the populations of catalytically active and inactive...

6. Exploring Ligand-Protein Interactions at Multiple Temperatures Using Macromolecular Crystallography

Chia-Ying HUANG (PSI - Paul Scherrer Institut)

05/06/2025, 11:30

Invited

Cryogenic temperatures may introduce artefacts that limit the understanding of protein dynamics, crucial to their biological functions. To address this, we developed a room-temperature (RT) X-ray crystallographic method that captures movie-like structural snapshots triggered by temperature [1]. This method revealed binding-mode changes of TL00150, a 175.15 Da fragment, in endothiapepsin....