Conveners
Radiation Damage in Temperature Controlled Crystallography
- Vincent Olieric (PSI - Paul Scherrer Institut)
Protein kinases are a large family of enzymes that regulate diverse cellular processes by transfering phosphate groups from ATP to their protein substrates. The enzymatic kinase domain (KD) represents a conserved fold, and KD phosphorylation, as well as interactions with cis-regulatory elements and other allosteric effectors, modulates the populations of catalytically active and inactive...
Cryogenic temperatures may introduce artefacts that limit the understanding of protein dynamics, crucial to their biological functions. To address this, we developed a room-temperature (RT) X-ray crystallographic method that captures movie-like structural snapshots triggered by temperature [1]. This method revealed binding-mode changes of TL00150, a 175.15 Da fragment, in endothiapepsin....
