Conveners
Pump-Laser Excitation Conditions in Time-Resolved Serial Femtosecond Crystallography
- Philip Johnson (PSI - Paul Scherrer Institut)
The high-intensity femtosecond pulses generated by X-ray free-electron lasers enable pump-probe studies of electronic- and nuclear changes during light-induced reactions. On time scales from femtoseconds to milliseconds and for a variety of biological systems, time-resolved serial femtosecond crystallography (TR-SFX) has provided detailed structural data on processes such as light-induced...
Time-resolved serial femtosecond crystallography (TR-SFX) enables the visualization of ultrafast structural changes in crystalline macromolecules [1]. For light-sensitive proteins, optical excitation lasers represent a convenient means to trigger the reaction. Generally, high excitation-laser fluences are used to maximize light-induced features in the Fourier difference electron density maps...
Time-resolved serial crystallography at X-ray Free Electron Lasers offers the opportunity to observe ultrafast photochemical reactions at the atomic level. The technique has yielded exciting molecular insights into various biological processes including light sensing and photochemical energy conversion. However, to achieve sufficient levels of activation within an optically dense crystal, high...
