Investigation of Ice-Binding Proteins using Heterodyne-Detected Vibrational Sum-Frequency Generation Spectroscopy

10 Jan 2018, 18:10
3h 5m
OASE (Paul Scherrer Institut)

OASE

Paul Scherrer Institut

Speaker

Ms Carolyn Moll (AMOLF)

Description

Antifreeze proteins (AFPs) and Antifreeze Glycoproteins (AFGPs) collectively abbreviated as AF(G)Ps are a unique class of proteins that modify ice crystal growth and enable the survival of organism in freezing and subfreezing habitats. The molecular working mechanism behind AF(G)Ps freezing inhibition is not well understood, because, as yet, there are few experimental techniques that allow obtaining molecular details on how antifreeze proteins function directly at the surface of ice. Here we use surface specific heterodyne-detected vibrational sum-frequency generation spectroscopy (HD-VSFG) to study the properties of antifreeze proteins directly at the ice surface and at the molecular level.

Significance statement

Antifreeze proteins (AFPs) and Antifreeze Glycoproteins (AFGPs) collectively abbreviated as AF(G)Ps are a unique class of proteins that modify ice crystal growth and enable the survival of organism in freezing and subfreezing habitats.

Primary author

Ms Carolyn Moll (AMOLF)

Co-authors

Prof. Athur DeVries (MBC) Prof. Huib Bakker (Amolf) Dr Konrad Meister (AMOLF)

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