Speaker
Mr
Marcel Petri
(Max Planck Institute for Biophysical Chemistry)
Description
Dronpa may be regarded as the prototype of photochromic proteins. These fluorescent proteins can be reversibly switched between a fluorescent bright and a non-fluorescent dark state by light excitation of the chromophore. Although the X-ray structure of Dronpa in both states has been determined in great detail little is known about the actual switching process itself.
We performed time-resolved SAXS experiments in solution to gain a detailed picture of the structural dynamics in this photo-switchable protein. For that purpose, our optical laser system has been attached to the cSAXS beamline and the optically induced changes have been probed by X-rays. Constructing three dimensional models from the data uncovered conformational alternations of the beta-can topology.
In this talk, I will report on these experiments and demonstrate the capabilities of time-resolved photo-SAXS techniques in soft condensed matter science.
Please specify poster or talk
talk
Please specify the session
Soft Condensed Matter
Primary author
Mr
Marcel Petri
(Max Planck Institute for Biophysical Chemistry)
