14–16 Oct 2020
Paul Scherrer Institut
Europe/Zurich timezone

Structural dynamics in proteins induced by and probed with X-ray free-electron laser pulses

15 Oct 2020, 13:30
20m
WBGB/019 (Paul Scherrer Institut)

WBGB/019

Paul Scherrer Institut

Forschungsstrasse 111 5232 Villigen PSI Switzerland

Speaker

Alexander Gorel (Max-Planck-Insitute for Medical Research Heidelberg)

Description

X-ray free-electron lasers (XFELs) enable crystallographic structure determination beyond the limitations imposed upon synchrotron measurements by radiation damage. The need for very short XFEL pulses can be relieved through gating of Bragg diffraction by loss of crystalline order as damage progresses but not if ionization events are spatially non-uniform due to underlying elemental distributions, as in biological samples. Indeed, correlated movements of iron and sulphur ions were observed in XFEL-irradiated ferredoxin microcrystals using unusually long pulses of 80 fs. Here we report a femtosecond time-resolved X-ray pump/X-ray probe experiment on protein nanocrystals. We observe changes in the protein backbone and aromatic residues as well as disulphide bridges. Simulations show that the latter’s correlated structural dynamics are much slower than expected for the predicted high atomic charge states due to the significant impact of ion caging and plasma electron screening. This indicates that dense-environment effects can strongly affect local radiation damage-induced structural dynamics.

Primary author

Alexander Gorel (Max-Planck-Insitute for Medical Research Heidelberg)

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