Speaker
Description
Robin Owen1, Danny Axford1, Ali Ebrahim1,2, Selina Storm1, Hiroshi Sugimoto3, Kensuke Tono3, Shigeki Owada3 Tadeo Moreno-Chicano2, Richard Strange2, Jonathan Worrall2 , Ivo Tews3 & Michael Hough2
1Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire, OX11 0DE, UK
2School of life Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK
3Spring8/SACLA, Harima Campus, Hyogo, Japan.
4Biological Sciences, University of Southampton, Institute for Life Sciences, Southampton, SO17 1 BJ, UK
robin.owen@diamond.ac.uk
The development of serial crystallography has been driven by XFELs but is now firmly established at high-brilliance synchrotron sources. Serial crystallography provides a means of efficiently collecting diffraction data from crystals held at ambient temperatures, providing access to more physiological environments and helping make structural transitions directly observable. Data collection at room temperature comes, however, with a price in the form of the rapid onset of radiation damage and greatly reduced crystal lifetimes. While this drawback can be addressed in part through the use of serial approaches, significant challenges for the experimenter remain.
I will describe fixed target serial delivery methods developed and implemented at SACLA and Diamond, illustrating the gains that can be realised using a multi-faceted approach at different, complementary, sources as well as some of the challenges both met and remaining.
