Success of crystallographic experiments using weakly diffracting macromolecular crystals depends on a proper choice of the data collection protocol. Radiation damage is a critical factor limiting data quality and being able to predict the absorbed dose and mitigate the effects of exposure to X-rays is very important.
We present the set of methods and programs intended for optimal planning of...
Recent brilliant micro-focused X-ray has enabled high resolution structural analyses of challenging biological proteins such from 10-µm sized crystals. One of the key technologies to achieve this is merging many partial datasets. This so-called ‘multiple small wedge data collection’ strategy was initially developed at Advanced Photons Source to accomplish data collection from in-meso phase...
