Speaker
Dr
Alexandre Mantion
(BAM Federal Institute for Materials Research and Testing)
Description
Silver nanoparticles belong to the most studied, produced and broadly distributed nanoparticles. Despite this, a critical investigation of their potential adverse effects on model living and ecological systems is still required. Along well-established biochemical tests, a reproducible and simple methodology to image their precise distribution in cells is missing. Their imaging in cells requires usually complex preparation like embedment/sectioning for electron microscopy or may have only a limited spatial resolution like in confocal Raman Microscopy. Often, studying the chemical composition and oxidation state of elements within a cell is a daring task. Our aim is thus to further exploit synchrotron radiation for nanotoxicology and contribute to develop complementary protocols for biological object imaging. To do this, we incubate a model macrophage cell line with our in house developed silver nanoparticles system and study the internal distribution of particles using ptychography, Scanning X Ray Transmission Microscopy (STXM), tomography and X ray absorption spectroscopy at the cSAXS and microXAS beamlines at SLS. We will present our first results in this poster.
Please specify the session
Imaging / Advancing Quantitative Chemical Imaging
Please specify poster or talk
poster
Primary author
Dr
Alexandre Mantion
(BAM Federal Institute for Materials Research and Testing)
Co-authors
Dr
Ana Diaz
(Paul Scherrer Institute)
Dr
Andrea Haase
(BfR - Federal Institute for Risk Assessment)
Dr
Andreas Luch
(BfR - Federal Institute for Risk Assessment)
Andreas Taubert
(Universität Potsdam and MPI Colloids and Interfaces)
Dr
Andreas Thünemann
(BAM Federal Institute for Materials Research and Testing)
Dr
Daniel Grolimund
(Paul Scherrer Institute)
Dr
Joan Vila
(Advanced Photon Source, Argonne)
Johanna Plendl
(BfR - Federal Institute for Risk Assessment)
Mrs
Monika Sachtleben
(Free University of Berlin, Department of Veterinary Medicine, Institute of Veterinary Anatomy)
Philipp Graf
(Universität Basel)
Wolfgang Meier
(Universität Basel)
